Purification of rat gamma globulin and the production of a specific antirat gamma globulin serum.
نویسندگان
چکیده
A method for the isolation of rat 7S gamma globulin based on multiple adsorptions with DEAE-Sephadex A-50 has been presented. The separation of rat 7S globulin requires a procedure modified from the one used for humans in terms of the buffer pH, and the number of adsorptions. Ultracentrifugal analysis indicates the resulting fraction is a single component. In addition, immunoelectrophoresis of the fraction when tested against anti-rat sera confirms the homogeneity of this 7S fraction. A monospecific antiserum to rat 7S gamma globulin has been produced. This antiserum is free of reaction with all other serum components when subjected to immunoelectrophoresis against normal rat serum.
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عنوان ژورنال:
- Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine
دوره 133 1 شماره
صفحات -
تاریخ انتشار 1970